Translational_Unit

Part:BBa_K497006

Designed by: Joanna Hare   Group: iGEM10_Macquarie_Australia   (2010-10-24)

Synechocystis heme oxygenase 1 with Agrobacterium tumefaciens bacteriophytochrome & RBS

This is an assembly of an Agrobacterium tumefaciens isolated bacteriophytochrome gene with a Synechocytis heme oxygenase 1 gene. A ribosome binding site has been added. The bacteriophytochrome gene produces a protein that interacts with Biliverdin, a chromophore. The chromophore is produced by heme oxygenase degradation of heme in the heme metabolic pathway. Sequence from National Center for Biotechnology Information

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2383
    Illegal PstI site found at 2779
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2383
    Illegal PstI site found at 2779
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2586
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2383
    Illegal PstI site found at 2779
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2383
    Illegal PstI site found at 2779
    Illegal NgoMIV site found at 1915
    Illegal NgoMIV site found at 2413
    Illegal AgeI site found at 2478
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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